Spitz nevus was first described by Sophie Spitz in 1948 as juvenile melanoma. The lesion is a benign melanocytic tumor, which consists of epiteloid- and spindelshaped cells. Histological is spitz nevus difficult to distinguish from malign melanomas and spitzoid melanomas. Loss of symmetry, loss of maturation in the deep component, nuclear polymorphism and hyper chromatic nucleus are features which can be found in melanomas. Some of these features are often seen in spitz nevus.

In couples diagnosed with unexplained infertility, the woman has normal ovulatory cycles and no organic pathology, and the man has normal semen. The period during which the implantation of an embryo can occur is defined as the window of implantation (WOI). For infertile women, disturbances in the endometrium maturation are hypothesized to impair the uterine receptivity for embryo implantaition. However, there are still no methods found to predict endometrial receptivity.Hemoglobin related-proteins hypothesized to be involved in embryo implantation have been found in the endometrium in both fertile and infertile women. The aim of this study was to determine whether there were differences in the expression of hemoglobin-related proteins, hemoglobin-?, cytoglobin, fetal hemoglobin and haptoglobin in the endometrium of fertile and infertile women.

Background. Immunohistochemistry is a useful method in the differential diagnosis between pleural mesotheliomas and metastatic adenocarcinomas in the pleura. Cytokeratin 20 and 7 have been used successfully as markers in studies determining primary location of adenocarcinomas from metastases. The current study is a complementary research of archived paraffininbedded material of cases with cancer origin. This study contributes a bigger statistical material that may facilitate the search for unknown primary site of adenocarcinoma by identification of metastatic cells in the pleura.Methods.

Syfte: Det har visat sig i tidigare studier att vissa kontaktlins- och linsvätskekombinationer ger mer påverkan på corneas yttre delar än andra kombinationer. Denna studie undersöker förekomsten av corneal staining med Extend® och Regard® vid användning av PureVision®, eftersom de linsvätskorna inte funnits med i tidigare större studier.Metod: Deltagarna använde Extend® till ena ögat, Regard® till andra och linserna PureVision®/Purevision® Toric, under tre veckors tid. Corneal staining mättes vid start, då deltagarna varit utan linser i tre dagar samt vid återbesök tre veckor senare. För att vara opartisk vid återbesöket var undersökaren inte medveten om vilken linsvätska deltagarna använde till vilket öga. Deltagarna fick även svara på några enkätfrågor angående komfort.Resultat: Extend® och Regard® gav liknande resultat i corneal staining.

This work is about collaboration and staining in the upper secondary school Electrical program. How should the coloring go to in order to increase students' motivation and thus facilitate students' learning is the main subject with the work. The study has shown that there is a functional co-operation today, and that students believe that if the cooperation would increase. It would also in-crease their motivation to improve..

The United Nations has, during many years, developed a model for a globally harmonized system for classification and labeling of chemicals, with the aim of it becoming a global standard. This system is implemented in the European Union through the CLP-regulation and is now working parallel to the existing directives until 1st of June 2015, when all the new classifications of substances and mixtures must be completed. The aim of this project was to adjust the laboratory to the new legislation, through inventory of stored chemicals, update the list of chemicals and perform self-classifications of mixtures. The work included handling of about 650 different chemicals with varying hazard classifications and search for information in corporate websites and chemical databases. This resulted in a new and complete list of chemicals stored and used in the laboratory and, in addition, an example of how to make a self classification of a mixture.

Syfte: Studiens syfte var att jämföra två linsrengöringsmetoders påverkan på hornhinnan. Studien jämförde tillverkarens rekommenderade användningsinstruktioner för Opti-Free® Express®, med den alternativa rengöringsmetoden att gnugga linserna, något som ofta rekommenderas av optiker.Metod: Deltagarna sågs vid två tillfällen för utvärdering av eventuell påverkan på hornhinnan. Värdena som återfanns vid första tillfället fungerade som ett utgångsvärde. Samtliga fynd dokumenterades, graderades och poängräknades efter vilken typ av staining samt utbredning. Cornea delades in i fem regioner; centralt, superiort, inferiort, nasalt och temporalt.

The dog has often served as a model animal for humans in scientific studies. This is due to the fact that dogs are susceptible to a wide range of diseases which also affect humans. One example is cancer, a condition that affects dogs as well as owners. Neoplastic diseases account for 18-23 % of all deaths in dogs (Bonnet et al., 2005 and Jagielski et al., 2002). Mammary tumor, or tumor of the breast, is the most common tumor form in intact dogs and women (Im et al., 2013). Mammary tumors originate in the udder, and are classified according to their histological characteristics.

Fertility is of central interest in the dairy production but has during the last decades declined. Increased milk yield has resulted in high pressure on the metabolism of the dairy cows that are supposed to manage the transition from dry cows to lactating cows within a few weeks around the parturition. Much indicate that metabolism and fertility are closely linked, with insulin playing a substantial part. There are many studies suggesting that the main part of gestation loss can be found during the early embryo development, a period which can be studied in vitro. The aim of this study was to test the effect of insulin during maturation in vitro and to evaluate two different fluorescent stainings on oocytes and embryos; a nuclear stain and a staining of apoptotic cells through the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-method. Bovine cumulus-oocyte-complexes (n=991) were aspirated from abattoir-derived ovaries.

Diabetes mellitus is a rising epidemic throughout the world and there is currently great interest in quantifying the beta-cell mass (BCM) in vivo non-invasively. In the present experiment, the feasibility of in vivo imaging of the glucagon-like peptide-1 receptor (GLP-1R) in beta-cells was examined, using the positron emission tomography (PET) tracer [68Ga]Ga-DO3A-VS-Cys40-exendin-4 as a marker, in native pancreatic beta-cells of a porcine diabetic animal model and healthy controls. Eight Swedish high-health domestic pigs were randomly assigned to be either controls or made diabetic using streptozotocin (STZ). The experiment proceeded during eight weeks, starting with an acclimatisation period. Once the pigs had been socialised they underwent surgery for the insertion of a jugular vein catheter, allowing induction of diabetes with STZ, intravenous (i.v.) injections and stress-free blood sampling. Development of diabetes was confirmed by clinical examinations, blood glucose values and insulin-staining of pancreatic sections post mortem. The diabetic pigs were insulin treated and responded well.

Syftet med denna studie var dels att undersöka användning av symptomfrågeformulär vid bedömning av ögontorrhet, dels att titta närmare på vilken metod och/eller vilka kombinationer av tester svenska optiker föredrar för bedömning av ögontorrhet.Metoder: Två olika typer av symptomfrågeformulär, den ena publicerad av The Canadian Dry Eye Epidemiology Society (CANDEES) och den andra med hjälp av Ocular Surface Disease Index (OSDI), översattes till svenska och distribuerades slumpmässigt bland patienter från en optikerpraktik i Kalmar. Dessutom har en webbaserad enkät skickats till 800 optiker för att undersöka deras förstahandsval av test för utvärdering av ögontorrhet. Deras andra, tredje och respektive fjärde val har också noterats, liksom eventuella ytterligare kommentarer.Resultat: Åttiofem patienter svarade på åtminstone ett av frågeformulären, varav 34% (17/50) CANDEES och 32,7% (16/49) OSDI respondenter uppgav att de hade milda till svåra symptom på torra ögon. Av de 800 e-postmeddelanden som skickades ut, svarade bara 64 (8%) optiker. Symptomfrågeformulär används oftast som enda testdiagnostik (42,2%).

Amyotrophic lateral sclerosis is one of the major neurodegenerative diseases, causing an ascending paralysis that usually kills the patient within a few years from disease onset. The motor neurons show aggregates of proteins which in approximately 20 % of cases of the familial form contain mutated SOD1 protein. Trehalose is a disaccharide which has been shown to reduce protein aggregation and increase viability in cell models and alleviate symptoms in animal models of several neurodegenerative diseases associated with protein aggregation. When given orally to the SOD1G93A mouse model of ALS, trehalose failed to slow down the disease progression, which has led to questions about the uptake and distribution of the molecule in this mouse strain. The aim of this study was to investigate whether significant levels of trehalose reach the central nervous system of the SOD1G93A mouse after oral administration. This was performed by a trehalose assay of the brain of trehalose treated animals. A glucose assay was optimised for use in small samples of brain lysate after the digestion of trehalose into glucose by trehalase, and the difference in glucose concentration before and after digestion represented the trehalose level.

This work is about collaboration and staining in the upper secondary school Electrical program. How should the coloring go to in order to increase students' motivation and thus facilitate students' learning is the main subject with the work. The study has shown that there is a functional co-operation today, and that students believe that if the cooperation would increase. It would also in-crease their motivation to improve..

Oxytocin is a hormone which plays a crucial role in many reproductive and behavioural functions. It affects many organs and the classical peripheral targets are the mammary glands during lactation and uterus during labour. Oxytocin receptors have recently been described in a variety of normal tissues and primary cell cultures, but also in neoplastic tissues and established neoplastic cell lines, as breast cancer cell lines. The signalling pathways and biological effects of the oxytocin/oxytocin receptor system seem to depend on species, type of tissue, physiological versus neoplastic state and receptor location within the cell membrane.The aim of this project was to find out if the cell proliferation in the canine mammary carcinoma cell line CMT-U27 was affected upon stimulation of oxytocin, and to investigate the presence of oxytocin receptors in these cells. The cell proliferation was examined by using an ELISA-kit, where the absorbance measured is relative to the amount of living cells.

The aim of this study was to isolate and identify Lactobacillus species indigenous to the gastrointestinal microflora of Swedish horses. These lactobacilli are to be used as probiotic candidates in research concerning antibiotic-associated diarrhoea in horses. Eight horses, age 6-10 years, were selected from a riding school for collection of faeces samples. Man Rogosa Sharp medium and gram staining were used for the selection of supposed lactobacilli. We could identify 33 isolates of supposed lactobacilli. With the use of API tests 22 of these 33 isolates could be identified.